To develop a real-time PCR method for detection of norovirus GⅡ in fresh strawberry, evaluate the application of magnetic beads enrichment method and PEG precipitation in detection of norovirus GⅡ and to detect norovirus GⅡ in 18 fresh strawberries from Beijing market.Methods Specific primers and probes were synthesized referenced to ISO/TS 15216-1. Magnetic beads and PEG precipitation were used to virus enrichment, and real-time PCR method was developed.Results The maximum recovery of magnetic beads enrichment and PEG methods were 1.730% (PBS buffer) and 1.682% (TGBE buffer) respectively. All 18 samples tested were negative.Conclusion The two virus enrichment methods developed in this study were suitable for the detection of norovirus GⅡ in fresh strawberry.