In recent years,pesticide residues in foods of animal origin have attracted wide attention;in particular,simultaneous detection of multiple pesticide residues has become a hot research subject at home and abroad.As a key step in the detection of pesticide residues,pretreatment of samples possesses a direct influence on the efficiency and accuracy of determination,and therefore,researchers have been searching for quick,high-efficient pretreatment techniques with small error and high recovery rate.Methods and research articles published in 1990-2011 on extraction,purification and detection of multi-pesticide residues in foods of animal origin were reviewed in this paper,and focusing on pretreatment methods and their future developments.