Objective Examining and analyzing the sensitivity of phage protein ligand assay to find out a more effective alternative method for detecting E.coli O157∶H7(VIDASECPT).Methods Detection Limits of VIDASECPT,VIDASECO and BAXO157 for E.coli O157∶H7 were determined by changing the concentration of E.coli O157∶H7 suspension;and comparative evaluation of VIDASECPT with conventional culture-based method for the detection of E.coli O157∶H7 in foods was conducted.Results E.coli O157∶H7 suspension at the level of 104 CFU/ml could be detected by VIDASECPT,however,the minimum concentration of E.coli O157∶H7 suspension that could be detected by the other two methods(VIDASECO and BAXO157) was 105 CFU/ml.There was no significant difference between VIDASECPT and conventional culture-based method for the detection of E.coli O157∶H7 in foods.Conclusion VIDASECPT is superior to the other two alternative methods for the detection of E.coli O157∶H7.