Objective To determine the distribution of tccP1 gene in Escherichia coli O157 isolates collected by the National Foodborn Disease Surveillance System from 2005 to 2007. Methods PCR was applied to detect the genes of 89 isolates of E. coli O157. Results The isolates carrying tccP1 accounted for 55.1%. Conclusion The tccP1 gene was widely present in O157:H7 strains but scarcely present in O157:hund/O157:NM strains. There were some associations of the distribution of tccP1 gene with eaeA gene but no association with shiga toxin gene carried by Escherichia coli O157.