Objective To explore the serotypes， drug-resistance phenotypes， molecular characteristics， and relevance of Vibrio parahaemolyticus （Vp） from aquatic and clinical isolates procured in 2020 from Shaoxing.Methods A total of 66 aquatic isolates （mainly soft-shelled turtles and spiral shells） and 67 clinical isolates of Vp were collected in 2020 from Shaoxing. Serotype detection was performed using slide agglutination. Drug susceptibility of nine kinds of antibiotics was acquired using the broth microdilution method. Clustering analysis was performed using pulsed field gel electrophoresis （PFGE）.Results The 66 aquatic isolates were divided into 9 serogroups and 35 serotypes. Moreover， O2：K28 was the dominant serotype， accounting for 18.18% （12/66）. The 67 clinical isolates were divided into 4 serogroups and 10 serotypes， and O3：K6 was the dominant serotype， accounting for 47.76% （32/67）. The aquatic isolates were resistant to nine kinds of antibiotics at different degrees， while the clinical isolates were only resistant to ampicillin. The resistance rate of aquatic isolates to ampicillin was 19.70% （13/66）， which was marginally higher than that of clinical isolates （11.94%， 8/67）， with two multidrug-resistant strains. A total of 52 aquatic isolates were divided into 51 band types and 50 band clusters. Moreover， 63 clinical isolates were divided into 32 band types and 17 band clusters. The 115 strains isolated from aquatic and clinical isolates were divided into 83 band types and 67 band clusters.Conclusion The Vp serogroups in aquatic isolates from soft-shelled turtles and spiral shells in Shaoxing are complex and diverse. The drug resistance of clinical isolates was lower than that of aquatic isolates. The similarity of molecular typing between aquatic isolates and clinical isolates was low， and there was no cross-aggregation. Therefore， the monitoring scope of aquatic products should be further expanded， the source of pollution more accurately determined， and the occurrence of foodborne diseases of Vp caused by aquatic products reduced.