QuEChERS结合高效液相色谱-串联质谱法测定禽蛋中的氯霉素药物残留
作者:
作者单位:

1.青岛海关技术中心,山东 青岛 266002;2.江南大学食品科学与技术学院,江苏 无锡 214122

作者简介:

赵飒 女 工程师 研究方向为食品质量安全检测 E-mail:425643339@qq.com

通讯作者:

张晓梅 女 高级工程师 研究方向为食品质量安全检测 E-mail:sdsywl@163.com

中图分类号:

R155

基金项目:

国家重点研发计划(2019YFC1605205);青岛海关科技项目(QK202017)


Determination of chloramphenicol residue in poultry eggs by QuEChERS combined with high performance liquid chromatography-tandem mass spectrometry
Author:
Affiliation:

1.Technology Center of Qingdao Customs District, Shandong Qingdao 266002, China;2.School of Food Science and Technology, Jiangnan University, Jiangsu Wuxi 214122, China

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    摘要:

    目的 建立QuEChERS(Quick、Easy、Cheap、Effective、Rugged、Safe)结合高效液相色谱-串联质谱法(HPLC-MS/MS)测定禽蛋(鸡蛋、鸭蛋、鹌鹑蛋、鹅蛋)中的氯霉素药物残留的分析方法。方法 禽蛋样品经乙腈提取,经(PSA∶C18=1∶1)净化处理,采用高效液相色谱-串联质谱法测定,同位素内标法定量。结果 禽蛋基质中氯霉素在0.020~2.0 μg/kg线性范围内,相关系数r>0.999;方法检出限0.020 μg/kg,定量限0.050 μg/kg;在添加浓度水平为0.020、0.10、0.50 μg/kg时,回收率范围为80.8%~119.0%,相对标准偏差为4.09%~15.21%(n=6)。采用建立的方法对市购禽蛋样品和天然污染鸡蛋样品进行验证测试,方法实用性满足残留测试要求。结论 本方法前处理操作简便、灵敏度高、可有效用于禽蛋中氯霉素药物残留的监控与定量测定。

    Abstract:

    Objective To develop a method for determination of chloramphenicol residue in poultry eggs (hen egg, duck egg, quail egg, and goose egg) using QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) protocols combined with high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS).Methods The chloramphenicol residue in egg samples was extracted with acetonitrile, followed by QuEChERS protocol with cleaning-up mixture (PSA∶C18=1∶1), and then determined by liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) with internal standard quantification.Results The linearity of chloramphenicol in poultry eggs was 0.020-2.0 μg/kg, with a correlation coefficient r>0.999. The limit of detection (LOD) of the method was 0.020 μg/kg, and the limit of quantitation (LOQ) was 0.050 μg/kg. Recoveries ranged from 80.8% to 119.0% at spiked level of 0.020, 0.10 and 0.50 μg/kg with the relative standard deviations (RSDs) of 4.09%-15.21% (n=6). The developed method was validated using real-life samples as well as naturally incurred egg samples and showed good applicability in chloramphenicol residue analysis in poultry eggs.Conclusion This proposed method is rapid, simple and sensitive, which could be effectively used for monitoring and determination of chloramphenicol residue in poultry eggs.

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赵飒,王培锋,聂晨,韩静静,邱芳,张晓梅,任燕杰,郭亚辉. QuEChERS结合高效液相色谱-串联质谱法测定禽蛋中的氯霉素药物残留[J].中国食品卫生杂志,2023,35(3):346-352.

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  • 收稿日期:2021-12-31
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  • 在线发布日期: 2023-05-24
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