Objective To give a solid foundation for the epidemiological data of diarrheagenic Escherichia coli （DEC）， and provide a basis for the rational drug use for foodborne diseases caused by DEC， the continuous monitoring of DEC from 2018 to 2020 in Liaoning Province was carried out. The pathogenic characteristics and drug sensitivity characteristics of DEC were investigated.Methods A total of 208 infant food in Liaoning Province were collected， from which Enterobacteriaceae was isolated， and 16S rRNA gene sequencing analysis and biochemical identification were carried out. The virulence genes of E.coli were detected. The serotype and drug resistance spectrum of EAEC were identified.Results Identified species of Enterobacteriaceae by 16S rRNA gene sequencing method was consistent with the biochemical results. The detection rate of EAEC in infant food was high， and the detection rate of virulence gene pic of EAEC was high. Virulence genotyping was consistent with the serum typing. According to the virulence gene carried by Escherichia coli， 25 strains of EAEC were detected， of which 40% of the strains （serotype O134：H9） carried virulence gene pic， 28% of the strains （serotype O3：H2） carried virulence gene aggR and astA， 16% of the strains （serotype O9：H6） carried virulence gene aggR， and 16% of the strains （serotype O62：H7） carried virulence gene astA. The carrying rate of EAEC virulence gene in 78 strains was 32.1%. The drug resistance of 25 EAEC strains was not optimistic， and there were multiple drug-resistant strains. The resistance was mainly for β lactams， macrolides， quinolones and tetracyclines.Conclusion EAEC was the main contamination of Escherichia coli in infant food in Liaoning Province， and had high drug resistance， which need more attention to be paid to.