Objective To detect the adulteration in milk and dairy products， the method for identification of animal derived components in milk and dairy products was constructed.Methods The specific primer probes of cattle， sheep， donkey and buffalo were designed and synthesized. The nucleic acid extraction method in milk and dairy products was optimized. The specificity， sensitivity and applicability of the designed fluorescent PCR method were verified.Results The method had strong specificity and sensitivity for common animal species. The detection sensitivities of cattle， sheep， donkey and buffalo were 0.001， 0.01， 0.01 and 0.01 ng， respectively.Conclusion The specificity， sensitivity， detection limit and other indicators of the constructed method can meet the needs of daily experiments， and provide strong technical support for supervision and testing.